Enzymes and Inhibitors
Sino Biological Oligo Substrates: METTL3/METTL14 Substrate
The synthetic single-stranded 27 base’s oligo ribo-nucleotide is used as a substrate for assay of methyltransferase METTL3/METTL14 complex.
Sino Biological Peptide Substrates: Histone H3 Peptide (1-21)
The Histone H3 Peptide (1-21) sequence (ARTKQTARKS-TGGKAPRKQLAGGKKC) is derived from human histone H3 (1-21) and is suitable for use as the substrate for histone methyltransferase (at K4 and K9) and acetyltransferase (at K9 and K14) family assays.
STA PHARMACEUTICAL US LLC Fmoc-D-2-Nitrophe | 10 g | CAS 478183-70-9 | MDL MFCD01317714
Fmoc-D-2-Nitrophe is a Amino Acid reagent (Subcategory: Phe) sold by WuXi TIDES. Offered in 10 g. Store at 4 °C. SDS available for reference.
Specifications
- CAS: 478183-70-9
- MDL: MFCD01317714
- InChIKey: KVLRWXBYPKSBFY-OAQYLSRUSA-N
- Molecular Weight: 432.432
- Molecular Formula: C24H20N2O6
- Purity: ≥95%
- Container Type: 60 mL HDPE
- Pack Size: 10 g
- Net Weight: 10 g
- Gross Weight: 25 g
- Commodity Code: 29242970
- Country Of Origin: China
- IUPAC: (R)-2-((((9H-fluoren-9-yl)methoxy)carbonyl)amino)-3-(2-nitrophenyl)propanoic acid
- SMILES: O=C([C@@H](CC1=CC=CC=C1[N+]([O-])=O)NC(OCC2C3=CC=CC=C3C4=CC=CC=C42)=O)O
Research Products International Corp 4-NITROPHENYL-A-D-MANNOPYRANOSIDE 500MG
Spectrophotometric substrate for detection of α-Mannosidase.
Free PNP: <200ppm
Cayman Chemical Pntothenate-AMC 5mg
A fluorogenic substrate for vanin-1; AMC is released upon enzymatic cleavage by vanin-1 and its fluorescence can be used to quantify vanin-1 activity; ex/em = 340-360/440-460 nm, respectively
LifeSensors Ubiquitin-AMC 50µg
Ubiquitin-AMC is prepared via the conjugation of 7-amino-4-methylcoumarin (AMC) to the C-terminus of mature ubiquitin leading to quenching of the intrinsic fluorescence of AMC. Upon incubation with a protease recognizing ubiquitin, such as USP2 or UCHL3, AMC is released and an increase in fluorescence can be measured using at 460 nm (380 nm excitation). The high purity of LifeSensors’ Ubiquitin-AMC results in improved signal to background ratios. This protein contains no extraneous tags.
Sino Biological Peptide Substrates: Amyloid Beta Peptide (1-42)
The Amyloid Beta Peptide (42 aa) sequence is DAEFRHDSGYEVHHQKLVFFAEDVGSNKGAIIGLMVGGVVIA. It is produced synthetically and treated with 1,1,1,3,3,3-Hexafluoro-2-propanol (HFIP) prior to drying which breaks down pre-formed fibrils and monomerizes the peptide.
Sigma Aldrich Fine Chemicals Biosciences Albumin–fluorescein isothiocyanate conjugate, MFCD00282182, 100 mg
Protein bovine, Synonym: FITC-albumin. Albumin−fluorescein isothiocyanate conjugate has been used: as a control to compare the intracellular delivery efficiency of proteins with or without carrier, for the fluorescent visualization of proteins and cells, as a blocking agent in ELISA, as a chemical for brain perfusion and fixation, for in vivo capillary–epithelial permeability experiment, and for confocal microscopy of right ventricular (RV) vasculature in rats.
List Biological Laboratories Inc SNAPtide Peptide Substrate, Fluorogenic FRET Technology, Botulinum Neurotoxin Detection, 200 nmoles
The SNAPtide Peptide Substrate (o-Abz/Dnp) is a synthetic peptide aimed at detecting Clostridium botulinum Type A Neurotoxin activity using advanced FRET technology. This product offers a unique advantage in its design, allowing for precise enzymatic activity measurement through the release of fluorophores upon cleavage. Supplied as a lyophilized powder, it is well-suited for research applications and guarantees a purity of ≥95%.
Medchemexpress LLC Her2/CD340 protein, rat (HEK293, His) | >95.0% | 5 UG
Recombinant rat HER2/CD340 protein expressed in HEK293 cells with a C-terminal His tag. Supplied as a purified ectodomain suitable for biochemical assays, antibody development, receptor binding studies, and structural or biophysical analysis. Molecular weight is approximately 91 kDa and purity is greater than 95% by reducing SDS-PAGE.
- Recombinant rat HER2 protein produced in HEK293 expression system.
- C-terminal His tag for efficient purification.
- High purity (>95%) as determined by reducing SDS-PAGE.
- Approximate molecular weight 91 kDa.
- Suitable for biochemical assays, antibody generation, and binding studies.
- Available in small research quantities (for example, 5 μg).
Sino Biological Peptide Substrates: SAMStide
The SAMStide peptide sequence (HMRSAMSGLHLVKRR) is based on the mouse acetyl-Coenzyme A carboxylase alpha (amino acid 73-85).
![Research Products International Corp NPG [4-Nitrophenyl-B-D-glucuronide], 1 Gram](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00006095-N12030-1.0.JPG-250.jpg)
Research Products International Corp NPG [4-Nitrophenyl-B-D-glucuronide], 1 Gram
Spectrophotometric substrate for detection of B-D-Glucuronidase.
Research Products International Corp P-NITROPHENYL-A-D-GLUCOPYRANOSIDE, 1 GRAM
Spectrophotometer substrate for Glucansucrases.
Free PNP: <250ppm
Abcam Carbo x yl-DCFDA (5(6)-Carbo x y-2',7'-dichlorofluorescein diacetate), Fluorogenic esterase substrate, 100MG
MW 529.28 Da, Purity >85%. Amine-reactive, fluorogenic, non-selective esterase substrate. Hydrolyses to DFCA (ab145439) fluorophore intracellularly. Labels intact cells. 2,7-Dichlorofluorescein (ab146332) derivative and DCFDA N-succinimidyl ester (ab145286) precursor.
The product is subject to the following: Abcam Restricted Use Statement
Enzo Life Sciences Ac-YVAD-AMC (5mg)
Fluorogenic caspase-1 substrate with a Km=14µM. Ex.:340-360nm, Em.:440-460nm.Sequence is based on precursor interleukin-1β Asp116 cleavage site for caspase-1. Alternative name: Caspase-1 substrate (fluorogenic). Formula: C33H39N5O10. MW: 665.7. Purity: ≥98% (HPLC). Appearance: White to off-white powder. Peptide content: 75-95%. Solubility: Soluble in DMSO. Dilute with distilled water or buffer, pH 7.0-7.5 (e. g. TRIS, HEPES). Long Term Storage: -20°C. Handling: Protect from light. Keep cool and dry.